The people of the EU have had a handful of years now to see what can happen to the economy in a large union. When one country stumbles, the entire economy of Europe goes down with it. The people of the UK figured this out and they voted for Brexit. Others will follow suit.
Yes, the recent vote in Italy a sign that the EU will not last. Based on the recent election of Donald Trump to president of the United States, as well as the even more pertinent Brexit vote, it seems like humanity is moving toward a more tribal and less global future.
The European Union is already on shaky ground after Britain's proposed plans to leave, and with other countries potentially following suit it signals a possible end to the EU. Considering the other members also, and how they have affected policies, it may signal a fresh start for Europe as a whole, each country independent but working together.
Yes, the referendum in Italy is only the latest sign that the EU will not last as it stands today. Voters voted against a more powerful government, and if people do not even want their own country having that much power it will be difficult to stay in a union where the government controls an entire continent.